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Thiocyanate hydrolase

From Wikipedia, the free encyclopedia
thiocyanate hydrolase
Identifiers
EC no.3.5.5.8
Databases
IntEnzIntEnz view
BRENDABRENDA entry
ExPASyNiceZyme view
KEGGKEGG entry
MetaCycmetabolic pathway
PRIAMprofile
PDB structuresRCSB PDB PDBe PDBsum
Gene OntologyAmiGO / QuickGO
Search
PMCarticles
PubMedarticles
NCBIproteins

A thiocyanate hydrolase (EC 3.5.5.8) is an enzyme belonging to the family of hydrolases. The systematic name of this enzyme class is thiocyanate aminohydrolase. This enzyme catalyzes the chemical reaction:

SCN + 2 H2O + H+ ⇌ SCO + NH3

The mechanism is proposed to involve a metal thiocyanate complex.

Structural studies

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As of late 2007, 4 structures have been solved for this class of enzymes, with PDB accession codes 2DD4, 2DD5, 2DXB, and 2DXC.

A second thiocyanate hydrolase with copper at its active site catalyzes its conversion to cyanate:[1]

SCN + H2O → OCN + H2S

References

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  1. ^ Tikhonova, Tamara V.; Sorokin, Dimitry Y.; Hagen, Wilfred R.; Khrenova, Maria G.; Muyzer, Gerard; Rakitina, Tatiana V.; Shabalin, Ivan G.; Trofimov, Anton A.; Tsallagov, Stanislav I.; Popov, Vladimir O. (2020). "Trinuclear Copper Biocatalytic Center Forms an Active Site of Thiocyanate Dehydrogenase". Proceedings of the National Academy of Sciences. 117 (10): 5280–5290. Bibcode:2020PNAS..117.5280T. doi:10.1073/pnas.1922133117. PMC 7071890. PMID 32094184.